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Banana (Musa spp.) as a model to study the meristem proteome: Acclimation to osmotic stress



  • Authors : Carpentier, S.; Witters, E.; Laukens, K.; Van Onckelen, H.; Swennen, R.; Panis, B.

  • Document type : Journal article

  • Year of publication : 2007

  • Journal title : Proteomics

  • Number : 7


  • Pages : 92-105

  • Peer-reviewed : Yes

  • ISSN : 1615-9853; 1615-9861

  • Language(s) : English

  • Abstract : Banana (Musa spp.) multiple shoot meristems are an excellent model to study the meristem proteome. Using a 2-DE protocol developed for small amounts of tissue and MS-based cross species polypeptide identification, we have revealed the meristem proteome and investigated the influence of sucrose-mediated osmotic stress in a dehydration-tolerant variety. Proteins that were significantly up- or down-regulated due to the high-sucrose treatment were classified using nonparametric univariate statistics. Our results suggest that the maintenance of an osmoprotective intracellular sucrose concentration, the enhanced expression of particular genes of the energyconserving glycolysis and the conservation of the cell wall integrity are essential to maintain homeostasis, to acclimate and to survive dehydration. By comparing the dehydration-tolerant variety with a dehydration-sensitive variety, we were able to distinguish several genotype-specific proteins (isoforms), and could associate the dehydration-tolerant variety with proteins involved in energy metabolism (e.g., phosphoglycerate kinase, phosphoglucomutase, UDP-glucose pyrophosphorylase) and proteins that are associated with stress adaptation (e.g., OSR40-like protein, abscisic stress ripening protein-like protein). This work shows that proteome analysis can be used successfully to perform quantitative difference analysis and to characterize genetic variations in a recalcitrant crop.

  • Keywords : MERISTEM CULTURE; TISSUE ANALYSIS; GENETIC VARIATION; PROTEINS; MERISTEMS; OSMOTIC STRESS

  • Open access : No

  • Document on publisher's site : close View article on publisher's site

  • Musalit document ID : IN070525

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