The use of 2D-electrophoresis and de novo sequencing to characterize inter- and intra-cultivar protein polymorphisms in an allopolyploid crop

• Authors : Carpentier, S.; Panis, B.; Renaut, J.; Samyn, B.; Vertommen, A.; Vanhove, A.C.; Swennen, R.; Sergeant, K.


• Document type : Journal article


• Year of publication : 2011


• Journal title : Phytochemistry


• Volume (number) : 72 (10)




• Pages : 1243-1250


• Peer-reviewed : Yes


• ISSN : 0031-9422


• Language(s) : English


• Abstract : Polyploidy and allopolyploidy have played an important role in the evolution of many plants and crops. Several techniques exist to characterize allopolyploid varieties. Analyzing the consequences of genomic reorganization at the gDNA level is a prerequisite but a better insight into the consequences for the phenotype is also primordial. As such, protein polymorphism analysis is important in understanding plant and crop biodiversity and is a driving force behind crop improvement. Our strategy to analyze protein isoforms and to detect possible gene silencing or deletion in bananas was based on protein analysis. Bananas are a good representative of a complex allopolyploid and important crop. We combined two-dimensional electrophoresis (2DE) and 2D DIGE with de novo MS/MS sequence determination to characterize a range of triploid varieties...


• Keywords : POLYPLOIDY; GENOMES; DNA; ELECTROPHORESIS; PROTEINS; GENETIC POLYMORPHISM; GENOMICS


• Open access : No


• Document on publisher's site : View article on publisher's site


• Musalit document ID : IN120110

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