Glucose performs better than fructose, sucrose, and diluted nectar for germination of banana pollen

• Authors : Waniale, A.; Mukasa, S.B.; Tugume, A.K.; Tumuhimbise, R.; Kubiriba, J.; Swennen, R.


• Document type : Conference paper


• Year of publication : 2020


• Conference : XXX International Horticultural Congress IHC2018: II International Symposium on Plant Breeding in Horticulture


• Book title : Acta Horticulturae 1282


• Publisher(s) : ISHS


• Place of publication : Leuven, Belgium


• Pages : 269-276


• Language(s) : English


• Abstract : Poor stigma receptivity is one of the limitations to seed set in East African Highland Bananas (EAHBs) that constrains their improvement through conventional breeding. This study aimed at identifying a suitable pollen germination media (PGM) for faster germination of banana pollen both in vitro and in vivo. PGM was prepared by dissolving 0.01 g H3BO3, 0.25 g MgSO4.7H2O, 0.25 g KNO3 and 0.4 g Ca(NO3)2 in 1 L along with different sugars. Sucrose, glucose, fructose, glucose + fructose, and glucose + fructose + sucrose at six concentration levels of 10, 30, 50, 100, 150 and 200 g/L were tested. Pollen from Calcutta 4 and TMB2x8075-7 diploid bananas was dusted on a cover slip and slowly lowered over four drops of PGM on a glass slide. The set up was incubated in a humid chamber for 3 h and germinated pollen counted using a light microscope at X40 magnification. Results revealed that glucose at 10 and 30 g/L concentration performed best with the latter having the higher pollen germination percentage. Results from comparison of 30 g/L glucose PGM with diluted banana nectar from Tereza and TMB2x8075-7 showed that 30 g/L glucose PGM generated highest pollen germination. It is suggested that glucose being a monosaccharide is a more readily available energy source for pollen. When 30 g/L glucose PGM was applied on stigmas after pollination, stigma receptivity was enhanced.


• Keywords : POLLEN; RTB; EAST AFRICAN HIGHLAND BANANAS; SEED SET


• Open access : No


• Document on publisher's site : View article on publisher's site


• Musalit document ID : IN200696

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