Cryopreservation of embryogenic tissues of Pinus nigra Arn. by a slow freezing method

• Authors : Salaj, T.; Panis, B.; Swennen, R.; Salaj, J.


• Document type : Journal article


• Year of publication : 2007


• Journal title : CryoLetters


• Volume (number) : 28 (2)




• Pages : 69-76


• Peer-reviewed : Yes


• ISSN : 0143-2044


• Language(s) : English


• Abstract : Six different embryogenic cell lines of Pinus nigra Arn. have been cryopreserved in liquid nitrogen using cryoprotection with sucrose (18 percent) and DMSO (7.5 percent). Post-thaw growth and tissue proliferation have been observed in five cell lines. The survival levels after storage in liquid nitrogen reached values between 62.5 and 100 percent. Growth of recovered embryogenic cells as well as somatic embryos is similar to the non-frozen tissues maintained in long-term culture. Somatic embryo maturation and plantlet regeneration occurred in all selected cell lines. (Author's abstract).


• Keywords : CULTURE MEDIUM; IN VITRO REGENERATION; SOMATIC EMBRYOGENESIS; CRYOPRESERVATION; PINUS NIGRA


• Open access : No


• Document on publisher's site : View article on publisher's site


• Musalit document ID : IN070492

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