Cryopreservation of the banana germplasm collection at the International Transit Centre - Bioversity International

• Authors : Panis, B.; Van Den Houwe, I.; Piette, B.; Swennen, R.


• Document type : Journal article


• Year of publication : 2007


• Journal title : Advances in Horticultural Science


• Volume (number) : 21 (4)




• Pages : 235-238


• Peer-reviewed : Yes


• ISSN : 0394-6169


• Language(s) : English


• Abstract : Cryopreservation is the ultimate method to preserve large collections of vegetatively propagated plant species, like banana, for the long term. Two cryopreservation protocols, i.e. vitrification of proliferating meristem clumps (called “scalps”) and vitrification of apical meristems were investigated. The protocols were successfully applied to 540 accessions belonging to 22 different banana groups. The regeneration rate after thawing strongly depends on the genomic group to which the accession belongs and the cryopreservation method that is used. For safe long-term storage, we opted for three independent successful experiments. An experiment is judged successful if at least one shoot from the stored material regenerates with more than 95% probability.


• Keywords : GERMPLASM; GENE BANKS; GERMPLASM COLLECTIONS; CRYOPRESERVATION


• Open access : No


• Musalit document ID : IN080504

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