Sugar-mediated acclimation: the importance of sucrose metabolism in meristems

• Authors : Carpentier, S.; Vertommen, A.; Swennen, R.; Witters, E.; Ferreira, C.F.; Souza Júnior, M.T.; Panis, B.


• Document type : Journal article


• Year of publication : 2010


• Journal title : Journal of Proteome Research


• Volume (number) : 9 (10)




• Pages : 5038-5046


• Peer-reviewed : Yes


• ISSN : 1535-3893; 1535-3907


• Language(s) : English


• Abstract : We have designed an in vitro experimental setup to study the role of sucrose in sugar-mediated acclimation of banana meristems using established highly proliferating meristem cultures. It is a first step toward the systems biology of a meristem and the understanding of how it can survive severe abiotic stress. Using the 2D-DIGE proteomic approach and a meristem-specific EST library, we describe the long-term acclimation response of banana meristems (after 2, 4, 8, and 14 days) and analyze the role of sucrose in this acclimation by setting up a control, a sorbitol, and a sucrose acclimation treatment over time. Sucrose synthase is the dominant enzyme for sucrose breakdown in meristem tissue, which is most likely related to its lower energy consumption. Metabolizing sucrose is of paramount importance to survive, but the uptake of sugar and its metabolism also drive respiration, which may result in limited oxygen levels...


• Keywords : SUCROSE; ADAPTATION; METABOLISM; MERISTEMS; STATISTICAL METHODS; CRYOPRESERVATION


• Open access : Yes


• Document on publisher's site : View article on publisher's site


• Musalit document ID : IN110148

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