Development of an efficient micropropagation technique for Musa cv. Udhayam (ABB)

• Authors : Saraswathi, M.S.; Praveena, S.; Uma, S.; Thangavelu, R.; Kannan, G.; Backiyarani, S.; Arivazhagan, T.


• Document type : Journal article


• Year of publication : 2014


• Journal title : Indian Journal of Horticulture


• Volume (number) : 71 (4)




• Pages : 452-457


• Peer-reviewed : Yes


• ISSN : 0972-8538, 0974-0112


• Language(s) : English


• Abstract : Experiments were conducted to devise an efficient method of micropropagation for a high yielding but recalcitrant banana cv. Udhayam (Pisang Awak, ABB) using shoot tip explants. Virus-indexed shoot tips were established in medium comprising full-strength Murashige and Skoog (MS) basal salts and vitamins, supplemented with 50 mg/l ascorbic acid, 100 mg/l myo-inositol and 4.0 mg/l benzylamino purine (BAP). Among the various media tested for shoot proliferation, MS medium with BAP (3.0/mg l) and 5% coconut water (CW) was found optimum as it produced the maximum number of 6.3 multiple shoots in a minimum period of 7.5 days. Rooting was achieved in the MS medium fortified with indole butyric acid (IBA) 0.5 mg/l + naphthalene acetic acid (NAA) 1.0 mg/l + activated charcoal (AC) 250 mg/l. Fully hardened planting material ensured high survival upon field transplantation. The development of a complete tissue culture protocol also includes the optimization of media and environment for successful primary and secondary hardening which has also been accomplished in the study.


• Keywords : MICROPROPAGATION; SHOOT TIPS; ORGANOGENESIS; PISANG AWAK ABB


• Open access : No


• Document on publisher's site : View article on publisher's site


• Musalit document ID : IN150116

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