A banana NAC transcription factor (MusaSNAC1) impart drought tolerance by modulating stomatal closure and H2O2 content

• Authors : Negi, S.; Tak, H.; Ganapathi, T.R.


• Document type : Journal article


• Year of publication : 2018


• Journal title : Plant Molecular Biology


• Volume (number) : 96 (4-5)




• Pages : 457-471


• Peer-reviewed : Yes


• ISSN : 0167-4412


• Language(s) : English


• Abstract : Drought is a abiotic stress-condition, causing reduced plant growth and diminished crop yield. Guard cells of the stomata control photosynthesis and transpiration by regulating CO₂ exchange and water loss, thus affecting growth and crop yield. Roles of NAC (NAM, ATAF1/2 and CUC₂) protein in regulation of stress-conditions has been well documented however, their control over stomatal aperture is largely unknown. In this study we report a banana NAC protein, MusaSNAC1 which induced stomatal closure by elevating H₂O₂ content in guard cells during drought stress. Overexpression of MusaSNAC1 in banana resulted in higher number of stomata closure causing reduced water loss and thus elevated drought-tolerance. During drought, expression of GUS (β-glucuronidase) under P MusaSNAC1 was remarkably elevated in guard cells of stomata which correlated with its function as a transcription factor regulating stomatal aperture closing. MusaSNAC1 is a transcriptional activator belonging to SNAC subgroup and its 5′-upstream region contain multiple Dof1 elements as well as stress-associated cis-elements. Moreover, MusaSNAC1 also regulate multiple stress-related genes by binding to core site of NAC-proteins CGT(A/G) in their 5′-upstream region. Results indicated an interesting mechanism of drought tolerance through stomatal closure by H₂O₂ generation in guard cells, regulated by a NAC-protein in banana.
  
  [Key message: MusaSNAC1 function in H₂O₂ mediated stomatal closure and promote drought tolerance by directly binding to CGT(A/G) motif in regulatory region of multiple stress-related genes.]


• Keywords : STOMATA; DROUGHT TOLERANCE; GENE REGULATION


• Open access : No


• Document on publisher's site : View article on publisher's site


• Musalit document ID : IN180133

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