Cryopreservation of banana meristem cultures: effect of vitrification solution on post-thaw survival in seven cultivars

• Chapter Authors : Agrawal, A.; Sharma, H.; Swennen, R.; Panis, B.


• Document type : Conference paper


• Year of publication : 2002


• Conference : Global Conference on Banana and Plantain, Bangalore (IND), 2002/10/28-31


• Book title : Global conference on banana and plantain: abstracts [Conférence globale sur les bananiers et bananiers plantain: résumés] [Conferencia global sobre banano y plátano : resúmenes]


• Editors : Singh, H.P.; Dadlani, N.K.


• Publisher(s) : AIPUB


• Place of publication : New Delhi (IND)


• Pages : 33-34


• Language(s) : English


• Abstract : The present work was carried out to determine the optimal PVS2 treatment time required for optimal post-thaw survival. Following a pre-treatment with 0.4 M sucrose for 2 weeks, proliferating meristem clumps of 7 cultivars were subjected to PVS2 for 30, 60, 90, 120 and 150 minutes at 0°C. The following cultivars, belonging to different genomics groups were tested: 'Cachaco' (AAB), 'Burro Cemsa' (AAB), 'Prata' (AAB), 'Obino l'Ewai' (AAB-plantain), 'Cemsa 3/4' (AAB-plantain), 'Guyod' (AA) and 'Kamarasange' (AB). For all genotypes tested, PVS2 did not prove to be toxic to the meristems (controls). In case of ABB bananas, controls remained almost 100 percent viable even up to 150 minutes. However in all others, viability decreased with increase in treatment time of PVS2.


• Keywords : MERISTEM CULTURE; FREEZING; MICROPROPAGATION; CULTURE MEDIUM; BIOLOGICAL PRESERVATION; MORTALITY; SURVIVAL; VITRIFICATION


• Open access : Yes


• PDF :


• Musalit document ID : IN030193

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