Ultrastructural changes associated with cryopreservation of banana (Musa spp.) highly proliferating meristems

• Authors : Helliot, B.; Swennen, R.; Poumay, Y.; Frison, E.A.; Lepoivre, P.; Panis, B.


• Document type : Journal article


• Year of publication : 2003


• Journal title : Plant Cell Reports


• Volume (number) : 21 (7)




• Pages : 690-698


• Peer-reviewed : Yes


• ISSN : 0721-7714; 1432-203X


• Language(s) : English


• Abstract : Cryopreservation has been shown to improve the frequency of virus elimination - specifically cucumber mosaic virus and banana streak virus - from banana (Musa spp.) plants. To understand the mode of action of cryopreservation for the eradication of viral particles, we examined the ultrastructure of meristem tips at each step of the cryopreservation process. Excised meristematic clumps produced from infected banana plants belonging to cv. 'Williams' (AAA, 'Cavendish' subgroup) were cryopreserved through vitrification using the PVS-2 solution. We demonstrated that the cryopreservation method used only allowed survival of small areas of cells in the meristematic dome and at the base of the primordia. Cellular and subcellular changes occurring during the cryopreservation process are discussed.


• Keywords : MERISTEM CULTURE; FREEZING; CULTURE MEDIUM; CUCUMBER MOSAIC VIRUS; SAFE VEGETAL MATERIALS; BIOLOGICAL PRESERVATION; BANANA STREAK VIRUS; CELL STRUCTURE; HISTOLOGY AND CITOLOGY; VITRIFICATION; TISSUE PROLIFERATION; ITC CODE


• Open access : No


• Document on publisher's site : View article on publisher's site


• Musalit document ID : IN030214

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