Cryopreservation of Musa suspension cultures and subsequent regeneration of plants

• Authors : Panis, B.; Withers, L.A.; De Langhe, E.A.L.


• Document type : Journal article


• Year of publication : 1990


• Journal title : CryoLetters


• Number : 11




• Pages : 337-350


• Peer-reviewed : Yes


• ISSN : 0143-2044


• Language(s) : English


• Abstract : Suspension cultures of musa have been preserved in liquid nitrogen and subsequenly regenerated using the following method : slow freezing at a rate of 1°C/min to -40°C followed by plunging the cultures into liquid nitrogen. Rapid thawing takes lace in a water bath at +40°C. Viability was determined by using the fluorescein diacetate (FDA) test and by regrowth on semi-solid medium. Of a rage of concentrations of dimethylsulphoxide (DMSD) and glycerol, DMSO at 5 percent gave the most effective cryopreservation, resulting in 64 percent regrowth of cells and cell clumps. Initiation of ice crystallization by seeding increased the regrowth rate to 92 percent. The FDA test revealed that only unorganiaed embryogenic aggregates survive freezing. regrown suspension cultures are regenerated into normal plants through somatic embryogenesis. (Author's Abstract).


• Keywords : CELL CULTURE; REGENERATION; SOMATIC EMBRYOGENESIS; CRYOPRESERVATION


• Open access : No


• Document on publisher's site : View article on publisher's site


• Musalit document ID : CA030360

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