Banana cell and tissue culture - review

• Chapter Authors : Strosse, H.; Van Den Houwe, I.; Panis, B.


• Document type : Conference paper


• Year of publication : 2004


• Book title : Banana improvement: cellular, molecular biology, and induced mutations


• Editors : Jain, S.M.; Swennen, R.


• Publisher(s) : Science Publishers


• Place of publication : Enfield (USA)


• Pages : 1-12


• Language(s) : English


• Abstract : The International Musa germplasm collection is sited at the INIBAP (International Network for the Improvement of Banana and Plantain) Transit Centre at K.U.Leuven. By now, more than 1000 different accessions of shoot-tip cultures have been initiated in vitro, multiplied and maintained at reduced temperature conditions (16+1°C). Shoot cultures are grown on MS (Murashige and Skoog) medium, supplemented with 30 g/l sucrose, 2.25 mg/l BA (6-benzyladenine) and 0.175 mg/l IAA (indole-3-acetic acid). In comparison with the culture medium on which shoot-tips are maintained, a tenfold decrease in cytokinin content (0.225 mg/l HA) induces regeneration of rooted plants. In contrast, adding 22.5 mg/l HA to the culture medium results in suppression of the apical dominance hi shoot-tip cultures and a reduction of corm and leaf tissue between meristematic tissue. Highly proliferating meristem cultures are obtained and used as starting material in the scalp methodology, the technique mass commonly applied for the development of embryogenic cell suspensions at the Laboratory of Tropical Crop Improvement, K.U.Leuvcn. Initiation and maintenance of cell cultures is rather labour intensive and lime consuming. However, since 1 ml of settled cells of a highly regenerable cell suspension can yield more than 100,000 plants, cell cultures are most suitable for mass clonal propagation. Moreover, embryogenic cell suspensions are highly preferred as target material for protoplast culture and genetic engineering since the risk of chimerism is circumvented because of the unicellular origin of regenerated plants. (Author's abstract).


• Keywords : TISSUE CULTURE; MICROPROPAGATION; BIOTECHNOLOGY; CULTURE MEDIUM; ADAPTATION


• Open access : Yes


• Document on publisher's site : View article on publisher's site


• Musalit document ID : IN040366

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