Characterization of Xanthomonas campestris pv. musacearum isolates: Causal agent of Enset bacterial wilt disease

• Authors : Bobosha, K.


• Document type : Thesis


• Diploma : Thesis (M.Sc., Biology, applied microbiology) Addis Ababa University


• Year of publication : 2003


• Publisher(s) : Addis Ababa University


• Place of publication : (ETH)


• Pages : 100


• Language(s) : English


• Abstract : Enset bacterial wilt disease is a major constraint in enset production system in Ethiopia. The causal agent for the disease is Xanthomonas campestris Pv. musacearum. Earlier, variation among isolates was observed in some preliminary laboratory and field experiments. However, further detailed study on variation of isolates was found necessary. Therefore, this study was conducted with the objective of detection of variation among isolates and evaluation of crude extracts from the plant Pychnostachis abyssinica against the isolates. Thirty-two bacterial samples were collected from 12 different enset growing weredas found in different enset growing zones of the Southern and Oromiya regions. Forty-eight different bacterial colonies were isolated and tested for their Gram-staining reaction and for their incapability of utilizing asparagine as a sole source of carbon and nitrogen. Isolates with negative gram staining reaction and those that did not grow on asparagine medium were subjected to biochemical, hypersensitivity and pathogenicity test. Following these tests, all isolates including those not able to grow on asparagine, those able to grow on asparagine and two reference strains (DSMZ 1050 and DSMZ 1350) were subjected to repetitive PCR analysis using ERIC1 and ERIC 2 primers. The different band patterns yielded from rep-PCR were analyzed using SPSS. Variations in reaction to different biochemical tests were observed among isolates. There were also isolates with similar reaction like YCMTge and LIHSsw2 from Yirgachefie and Limo and FGMTge and YCMSas from Fisseha genet and Yirgachefie, respectively. Pathogenicity test was found negative to all, which needs further investigation on the loss of the virulence of the bacterium on the host plant. The results from discriminant function analysis, principal component analysis and cluster analysis based on the band patterns of the isolates resulted from repetitive PCR showed variation and similarities among isolates.


• Keywords : BACTERIA; ISOLATION TECHNIQUES; IDENTIFICATION; PESTS; ENSET; XANTHOMONAS CAMPESTRIS PV. MUSACEARUM; ETHIOPIA


• Open access : Yes


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• Musalit document ID : ET050012

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