Development of embryogenic cell suspensions from shoot meristematic tissue in bananas and plantains (Musa spp.)

• Authors : Strosse, H.; Schoofs, H.; Panis, B.; Andre, E.; Reyniers, K.; Swennen, R.


• Document type : Journal article


• Year of publication : 2006


• Journal title : Plant Science


• Volume (number) : 170 (1)




• Pages : 104-112


• Peer-reviewed : Yes


• ISSN : 0168-9452


• Language(s) : English


• Abstract : Multiple meristem cultures of 18 varieties belonging to 5 genome types in Musa (AA, AAA, AAA-h, AAB and ABB) were established by culturing elongated shoots on MS medium supplemented with 100 mM BAP. The top layers comprising the most meristematic tissue, i.e. scalps, were excised and induced for embryogenesis on media containing 1-50 mM 2,4-dichlorophenoxyacetic acid (2,4-D). Embryogenic responses were obtained for each of the tested concentrations, with an optimum at 5 mM 2,4-D. From the 24,375 scalps tested, only 3.3 percent resulted in an embryogenic response. The average embryogenic frequency was 6.0 percent for cooking bananas (ABB), 3.8 percent for Cavendish-type bananas (AAA) and 1.8 percent for plantains (AAB). Once embryogenic complexes were transferred to liquid maintenance medium, embryogenic cell suspensions with high regeneration capacity were obtained. (Author's abstract).


• Keywords : MERISTEM CULTURE; CULTURE MEDIUM; 2,4-D; PLANT GROWTH SUBSTANCES; BA; GENOTYPES; REGENERATION; SOMATIC EMBRYOGENESIS; ITC CODE


• Open access : No


• Document on publisher's site : View article on publisher's site


• Musalit document ID : IN060051

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